Experiment

Experiment
A.002

Experiment
Fertilisation and cytoplasmic reorganisation in amphibian fixing, the development of Xenopus laevis eggs fertilised in microgravity.

Primary investigator
G.A. Ubbels

Co-investigator
T. Brom, J.M. Narraway, W. Berendsen, S. Kerkvliet

Contact point
Hubrecht Laboratory (NIOB)

Category
Life Sciences

Main research area
Developmental Biology

Keywords
Automatic Experiment Container
Biorack
Dorso-ventral polarity
Xenopus leavis
Egg preservation

Abstract
During the TEXUS-17 sounding-rocket flight, 8 containers, each loaded with 30 eggs and a testis and different salt solutions, were activated in a specially constructed air-tight, aluminium box, which provided perfect insulation during the experiment. .... (full text)

Objectives
Investigation of the role of gravity in determination of the dorso-ventral axis in the developing embryo of Xenopus Laevis.

Reference
ESA E507

Subject of investigation
Process research

Subject name
Amphibian egg fertilisation

Subject description
Xenopus laevis

Specific method
Automatic Experiment Container

Results
A first analysis has revealed various structures on the animal cortex, which, according to the literature, can be assumed to be connected with sperm penetration in various stages of development. This conclusion is further substantiated by a light microscopic analysis of .... (full text)

Funding agency
NIVR, SRON

Launcher
Texus sounding rocket

Mission
Texus-17

Facility
TEM-06-15

Duration
403 seconds (microgravity time)

Equipment
Details of Experiment Container

Success
Yes, first fertilisation of Xenopus eggs under microgravity circumstances.

Video
Embryonic development of Xenopus Leavis

General
Related experiments:
On STS 61A (1985)
On Maser-3 (1989)
On IML-1 (1992)


Extracted from a prototype of the Dutch Microgravity Compact Disc