Experiment

Experiment

A.014

Experiment

Cell Fusion

Primary investigator

Jongkind

Co-investigator

Verkerk, A

Contact point

Erasmus University of Rotterdam

Category

Life Science

Main research area

Cell Fusion

Sub research areas

Cell-cell interaction

Keywords

Electrofusion; Cytoskeleton

Abstract

Microgravity has no effect on cell fusion

Objectives

Study of the effect of microgravity on membrane functions

Subject of investigation

Membrane fusion
Electrofusion apparatus

Subject name

Human fibroblasts

Standard method

Cell culture
Electromicroscopy
Electro fusion
Cell staining

Specific method

Automatic change of solutions in EPBU

Experiment procedure

Cell culture
Electrofusion
Cell culture
Cell staining

Results

Microgravity has no effec on cell fusion and cytoskeleton

Experiment

CIS-3 Fusion

Funding agency

ESA

Launcher

Maser sounding rocket

Mission

Maser-5

Facility

Cells In Space (CIS)

Duration

428 sec

Equipment

EPBU

Success

Yes

Note

Technically the experiment was a succes; It give a clear answer

Operational Conststraints

Microgravity < 0.01g
Culture medium
High resistance medium
Temperature 37 degrees Celcius